Tracking and Post-Sequence Processing Data from Licor
Written by Eda Malaj
Updated 08-23-00
  1. Open the LICOR e-Seq program
  2. Click File, go to Open Run and open the run from the scanner or the file in the computer.
  3. Go to Analysis on the icons at the top of the gel image and open the Sequence Wizard.
    1. Press the Modify button and check that there are 16 samples to be analyzed.
    2. Even if you have less than 16 samples please put 16.
    3. Save the changes that you made and go back to Sequencing Wizard window.
  4. If you wish to not analyze a sample or lane, you must have the Sequence Wizard window open.
    1. Then, Alt-Click on the green sample marker on the gel image window.
    2. The green sample marker will turn red, the green line marker will turn yellow, and this sample will not be analyzed.
  5. After you have chosen the samples you would like to sequence, click Done in the Sequence Wizard window.
  6. Clicking Done then opens the Sequencing Status window, clicking Next opens the Post Sequencing Wizard that is used to specify post sequencing action. The Sequencing Status window also can be opened by selecting Sequencing Status on the Analysis menu.
  7. Clicking Start on the Sequencing Status window will initiate the lane finding.
  8. After the lanes are tracked, the Edit Lanes window will open.
  9. A vertical lane line is displayed in the center of each lane on the image.
    1. Lane makers lines can be selected and moved if some of the markers are not centered in the lanes.
    2. When the lanes are properly positioned, click OK.
    3. Press Continue in the Sequencing Status window to continue with the sequence analysis.
    4. After completion, the Cancel button on the right side of the Sequencing Status window changes to Done.
    5. Click Done to finish the analysis.
  10. Open the Post Sequencing Wizard from the Analysis menu to open the Post Sequencing Step 1 Window.
  11. Then, Click the Next button to open the Post Sequencing Step 2 Window which will allow you to export the data to a specific folder.
  12. To export the data, go to export SCF Curve 2.0 and press Done.
  13. Your data are now in the specific folder you choose above, and the data can be transfered to the abidata/abi directory on the Sun computers by FTP.
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