Table 5: Summary of the values for each fold coverage for a 50 Kbp to 500Mb
sequencing target with an average read length of 100 or 200 bases
50 Kbp Cosmid (G=50,000) Read Length 100 bases Read Length 200 bases
From Table 2: From Table4: Gap Length/ From Table4: Gap Length/
fold Total bases total gap length Number of # gaps= Number of # gaps= %
coverage sequenced e-c in bases =Ge-c Gaps = Ne-c # bases per gap Gaps = Ne-c # bases per gap complete
1 50,000 0.37 18,500 185.0 100.0 92.5 200.0 63
2 100,000 0.135 6,750 135.0 50.0 67.5 100.0 87.5
3 150,000 0.05 2,500 75.0 33.3 37.5 66.7 95
4 200,000 0.018 900 36.0 25.0 18.0 50.0 98.2
5 250,000 0.0067 335 16.8 20.0 8.4 40.0 99.4
6 300,000 0.0025 125 7.5 16.7 3.8 33.3 99.75
7 350,000 0.0009 45 3.2 14.3 1.6 28.6 99.91
8 400,000 0.0003 15 1.2 12.5 0.6 25.0 99.97
9 450,000 0.0001 5 0.5 11.1 0.2 22.2 99.99
10 500,000 0.000045 2 0.2 8.9 0.1 17.8 99.995
150 Kbp BAC (G=150,000) Read Length 100 bases Read Length 200 bases
From Table 2: From Table4: Gap Length/ From Table4: Gap Length/
fold Total bases total gap length Number of # gaps= Number of # gaps= %
coverage sequenced e-c in bases =Ge-c Gaps = Ne-c # bases per gap Gaps = Ne-c # bases per gap complete
1 150,000 0.37 55,500 555 100 278 200 63
2 300,000 0.135 20,250 405 50 203 100 87.5
3 450,000 0.05 7,500 225 33 113 67 95
4 600,000 0.018 2,700 108 25 54 50 98.2
5 750,000 0.0067 1,005 50 20 25 40 99.4
6 900,000 0.0025 375 23 17 11 33 99.75
7 1,050,000 0.0009 135 9 14 5 29 99.91
8 1,200,000 0.0003 45 4 13 2 25 99.97
9 1,350,000 0.0001 15 1 11 1 22 99.99
10 1,500,000 0.000045 6 1 9 0 18 99.995
300 Kbp Target Genome Read Length 100 bases Read Length 200 bases
From Table 2: From Table4: Gap Length/ From Table4: Gap Length/
fold Total bases total gap length Number of # gaps= Number of # gaps= %
coverage sequenced e-c in bases =Ge-c Gaps = Ne-c # bases per gap Gaps = Ne-c # bases per gap complete
1 300,000 0.37 111,000 1,110 100 555 200 63
2 600,000 0.135 40,500 810 50 405 100 87.5
3 900,000 0.05 15,000 450 33 225 67 95
4 1,200,000 0.018 5,400 216 25 108 50 98.2
5 1,550,000 0.0067 2,010 101 20 50 40 99.4
6 1,800,000 0.0025 750 45 17 23 33 99.75
7 2,100,000 0.0009 270 19 14 9 29 99.91
8 2,400,000 0.0003 90 7 13 4 25 99.97
9 2,700,000 0.0001 30 3 11 1 22 99.99
10 3,000,000 0.000045 12 1 9 1 18 99.995
2 Mbp Bacterial Genome Read Length 100 bases Read Length 200 bases
From Table 2: From Table4: Gap Length/ From Table4: Gap Length/
fold Total bases total gap length Number of # gaps= Number of # gaps= %
coverage sequenced e-c in bases =Ge-c Gaps = Ne-c # bases per gap Gaps = Ne-c # bases per gap complete
1 2,000,000 0.37 740,000 7,400 100 3,700 200 63
2 4,000,000 0.135 270,000 5,400 50 2,700 100 87.5
3 6,000,000 0.05 100,000 3,000 33 1,500 67 95
4 8,000,000 0.018 36,000 1,440 25 720 50 98.2
5 10,000,000 0.0067 13,400 670 20 335 40 99.4
6 12,000,000 0.0025 5,000 300 17 150 33 99.75
7 14,000,000 0.0009 1,800 126 14 63 29 99.91
8 16,000,000 0.0003 600 48 13 24 25 99.97
9 18,000,000 0.0001 200 18 11 9 22 99.99
10 20,000,000 0.000045 90 9 10 5 20 99.995
4 Mbp Bacterial Genome Read Length 100 bases Read Length 200 bases
From Table 2: From Table4: Gap Length/ From Table4: Gap Length/
fold Total bases total gap length Number of # gaps= Number of # gaps= %
coverage sequenced e-c in bases =Ge-c Gaps = Ne-c # bases per gap Gaps = Ne-c # bases per gap complete
1 4,000,000 0.37 1,480,000 14,800 100 7,400 200 63
2 8,000,000 0.135 540,000 10,800 50 5,400 100 87.5
3 12,000,000 0.05 200,000 6,000 33 3,000 67 95
4 16,000,000 0.018 72,000 2,880 25 1,440 50 98.2
5 20,000,000 0.0067 26,800 1,340 20 670 40 99.4
6 24,000,000 0.0025 10,000 600 17 300 33 99.75
7 28,000,000 0.0009 3,600 252 14 126 29 99.91
8 32,000,000 0.0003 1,200 96 13 48 25 99.97
9 36,000,000 0.0001 400 36 11 18 22 99.99
10 40,000,000 0.000045 180 18 10 9 20 99.995
20 Mbp Fungal Genome Read Length 100 bases Read Length 200 bases
From Table 2: From Table4: Gap Length/ From Table4: Gap Length/
fold Total bases total gap length Number of # gaps= Number of # gaps= %
coverage sequenced=G e-c in bases =Ge-c Gaps = Ne-c # bases per gap Gaps = Ne-c # bases per gap complete
0.5 10,000,000 0.61 6,100,000 61,000 100 30,500 200 39
1 20,000,000 0.37 7,400,000 74,000 100 37,000 200 63
1.5 30,000,000 0.22 6,600,000 66,000 100 66,000 100 77.7
2 40,000,000 0.135 2,700,000 54,000 50 27,000 100 87.5
3 60,000,000 0.05 1,000,000 30,000 33 15,000 67 95
4 80,000,000 0.018 360,000 14,400 25 7,200 50 98.2
5 100,000,000 0.0067 134,000 6,700 20 3,350 40 99.4
6 120,000,000 0.0025 50,000 3,000 17 1,500 33 99.75
7 140,000,000 0.0009 18,000 1,260 14 630 29 99.91
8 160,000,000 0.0003 6000 480 13 240 25 99.97
9 180,000,000 0.0001 2000 180 11 90 22 99.99
10 200,000,000 0.000045 900 90 10 45 20 99.995
40 Mbp Fungal Genome Read Length 100 bases Read Length 200 bases
From Table 2: From Table4: Gap Length/ From Table4: Gap Length/
fold Total bases total gap length Number of # gaps= Number of # gaps= %
coverage sequenced=G e-c in bases =Ge-c Gaps = Ne-c # bases per gap Gaps = Ne-c # bases per gap complete
0.5 20,000,000 0.61 12,200,000 122,000 100 61,000 200 39
1 40,000,000 0.37 14,800,000 148,000 100 74,000 200 63
1.5 60,000,000 0.22 13,200,000 176,000 75 66,000 200 77.7
2 80,000,000 0.135 5,400,000 162,000 33 54,000 100 87.5
3 120,000,000 0.05 2,000,000 80,000 25 30,000 67 95
4 160,000,000 0.018 720,000 36,000 20 14,400 50 98.2
5 200,000,000 0.0067 268,000 16,080 17 6,700 40 99.4
6 240,000,000 0.0025 100,000 7,000 14 3,000 33 99.75
7 280,000,000 0.0009 36,000 2,880 13 1,260 29 99.91
8 320,000,000 0.0003 12,000 1,080 11 480 25 99.97
9 360,000,000 0.0001 4000 400 10 180 22 99.99
10 400,000,000 0.000045 1800 180 10 90 20 99.995
500 Mbp Eukaryotic Genome Read Length 100 bases Read Length 200 bases
From Table 2: From Table4: Gap Length/ From Table4: Gap Length/
fold Total bases total gap length Number of # gaps= Number of # gaps= %
coverage sequenced=G e-c in bases =Ge-c Gaps = Ne-c # bases per gap Gaps = Ne-c # bases per gap complete
0.5 250,000,000 0.61 152,500,000 1,525,000 100 762,500 200 39
1 500,000,000 0.37 152,500,000 1,850,000 82 925,000 165 63
1.5 750,000,000 0.22 165,000,000 1,650,000 100 825,000 200 77.7
2 1,000,000,000 0.135 5,400,000 1,350,000 4 675,000 8 87.5
3 1,500,000,000 0.05 2,000,000 750,000 3 375,000 5 95
4 2,000,000,000 0.018 720,000 360,000 2 180,000 4 98.2
5 2,500,000,000 0.0067 268,000 167,500 2 83,750 3 99.4
6 3,000,000,000 0.0025 100,000 75,000 1 37,500 3 99.75
7 3,500,000,000 0.0009 36,000 31,500 1 15,750 2 99.91
8 4,000,000,000 0.0003 12,000 12,000 1 6,000 2 99.97
9 4,500,000,000 0.0001 4000 4,500 1 2,250 2 99.99
10 5,000,000,000 0.000045 1800 2,250 1 1,125 2 99.995