Sequencing Genomic Clones from Chlamydomonas reinhardtiiat the University of Oklahoma
Chlamydomonas reinhardtii is a unicellular green alga which
swims with two flagella and has chloroplasts for photosynthesis.
- The genome is ~100 Mbp and is distributed in 17 chromosomes.
- The initial goal of the Chlamydomonas reinhardtii genome
project is to generate EST sequences, and to date there are
over 55,860 Chlamydomonas ESTs in GenBank.
- Because Chlamydomonas reinhardtii is quite GC rich
we have begun a pilot project to sequence several BACs
in collaboration with
Dr. Susan K. Dutcher
in the Department of Genetics
at Washington University School of Medicine in St. Louis, Missouri.
These BAC clones cover a region of ~600 kbp in the Chlamydomonas reinhardtii genome.
- The BAC library prepared by Pete Lefebvre in collaboration with Genome
Systems (now IncyteGenomics) is available from IncyteGenomics.
- Requests for these BACs should be directed to
- A table viewing the BAC clones being sequenced can
be viewed at URL:
- In accord with the Bermuda Agreement and NHGRI policy, we are depositing all our human
genomic sequence data into the
High-Throughput Genomic Sequences (HTGS) division of GenBank
as soon as a target large insert clone sequencing project can be assembled
into contigs greater than 2 kb.
The following BAC clones are being sequenced.
This sequence data has been deposited into GenBank, and given accession numbers:
- cr-1j6 -
- cr-32m22 -
- cr-3h1 -
- cr-40a20 -
- cr-19b23 -
- cr-4i21 -
- Search Using Blastall on the OU Server.
Chlamydomonas reinhardtii Related Links:
Should you find this data useful and wish to reference it, please acknowledge
- Susan K. Dutcher, Andrea M. Preble and Craig Rackley in the Department of Genetics at Washington University
School of Medicine in St. Louis, Missouri and
- Bruce A. Roe, Shaoping Lin, Hongui Jia and HongMin Wu at the Advanced
Center for Genome Technology, Department of Chemistry and Biochemistry,
The University of Oklahoma, Norman, Oklahoma.
Bruce Roe, email@example.com