Sequencing Genomic clones from Chlamydomonas reinhardtii at the University of Oklahoma

Sequencing Genomic Clones from Chlamydomonas reinhardtiiat the University of Oklahoma

Chlamydomonas reinhardtii is a unicellular green alga which swims with two flagella and has chloroplasts for photosynthesis.

The genome is ~100 Mbp and is distributed in 17 chromosomes.

The initial goal of the Chlamydomonas reinhardtii genome project is to generate EST sequences, and to date there are over 55,860 Chlamydomonas ESTs in GenBank.

Because Chlamydomonas reinhardtii is quite GC rich (~65% G+C), we have begun a pilot project to sequence several BACs in collaboration with Dr. Susan K. Dutcher in the Department of Genetics at Washington University School of Medicine in St. Louis, Missouri. These BAC clones cover a region of ~600 kbp in the Chlamydomonas reinhardtii genome.

The BAC library prepared by Pete Lefebvre in collaboration with Genome Systems (now IncyteGenomics) is available from IncyteGenomics.

Requests for these BACs should be directed to Incyte Genomics

A table viewing the BAC clones being sequenced can be viewed at URL: http://www.genome.ou.edu/chlamy_totals.html

In accord with the Bermuda Agreement and NHGRI policy, we are depositing all our human genomic sequence data into the High-Throughput Genomic Sequences (HTGS) division of GenBank as soon as a target large insert clone sequencing project can be assembled into contigs greater than 2 kb.
The following BAC clones are being sequenced. This sequence data has been deposited into GenBank, and given accession numbers:

cr-1j6 - AC090434
cr-32m22 - AC090435
cr-3h1 - AC090436
cr-40a20 - AC087726
cr-19b23 - AC090433
cr-4i21 - AC091776

Search Using Blastall on the OU Server.

Chlamydomonas reinhardtii Related Links:

Chlamydomonas Web Resources from Wallace Marshall at Yale University
Chlamydomonas genome project
JGI Chlamydomonas reinhardtii whole genome shotgun sequencing

Acknowledgment

Should you find this data useful and wish to reference it, please acknowledge

Susan K. Dutcher, Andrea M. Preble and Craig Rackley in the Department of Genetics at Washington University School of Medicine in St. Louis, Missouri and
Bruce A. Roe, Shaoping Lin, Hongui Jia and HongMin Wu at the Advanced Center for Genome Technology, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma.

Bruce Roe, broe@ou.edu