Cryptococcus neoformans cDNA Sequencing


Cryptococcus neoformans is an encapsulated yeast that infects the human host via the respiratory tract where it usually causes an inapparent infection. In the susceptible host, it may disseminate, typically producing a chronic and life-threatening meningitis. The Cryptococcus neoformans serotypes A and D are responsible for the overwhelming majority of pulmonary infections in AIDS patients.


Cryptococcus neoformans strain H99 Latest Data Release - May 19, 2004



Cryptococcus neoformans strain B3501 Latest Data Release - May 19, 2004

  • The Cryptococcus neoformans strain B3501 EST's are being generated by Doris Kupfer, Heather Bell, Sunkyoung So, Yuong Tang, Sara Downard, Laura Hern and Ging Sobharaksha in collaboration with Juneann Murphy and Dave Dyer at the OU-Health Sciences Center, and Kent Buchanan at the Tulane University Medical School.
  • We earlier end sequenced all available templates (ca. 4000 reactions) from both ends of the directionally cloned inserts after excision into pBlueScript SK- and now have end sequenced an additional 1700 cDNAs to obtain ca. 3300 ESTs from c. neo gwown in Low Iron Media.
  • All of our data be available from our ftp site, and we will add the ability to perform blast searches on this data. A keyword search of a blastx search of GenBank with this data also will be available.

Cryptococcus neoformans strain 184A Latest Data Release - May 19, 2004

  • The Cryptococcus neoformans strain 184A EST's are being generated by Doris Kupfer and Sunkyoung So in collaboration with Juneann Murphy and Dave Dyer at the OU-Health Sciences Center, and Kent Buchanan at the Tulane University Medical School.
  • We now have end sequenced all available templates (ca. 2525 reactions) from both ends of the directionally cloned inserts after excision into pBlueScript SK-.
  • All of our data be available from our ftp site, and we will add the ability to perform blast searches on this data. A keyword search of a blastx search of GenBank with this data also will be available.

Search the Cryptococcus neoformans cDNA sequence data



Obtaining the sequence data via ftp

All of the cDNA sequence data we have obtained to date is available from our ftp site directly, by anonymous ftp to ftp.genome.ou.edu (logon as anonymous with password your e-mail address), cd to /pub/cneo and get the ascii data file called cneo_est_with_anno.fa or cneo_est_wo_anno.fa. These files contains all the EST's sequenced to date concatenated into a flat file either with or without annotation, respectively.

  • Cryptococcus neoformans strain H99 Sequencing Data via ftp
  • Cryptococcus neoformans strain B3501 Sequencing Data via ftp
  • Cryptococcus neoformans strain 184A Sequencing Data via ftp

    Clarification of our naming convention

    For Cryptococcus neoformans
    • The 3' end sequences have the notation .f1 at the end of the clone name
    • The 5' end sequences have the notation .r1 at the end of the clone name


    Clone Availability

    Many of cDNAs representing individual ESTs have been deposited at the Fungal Genetics Stock Center and they should be contacted should you be interested in obtaining any of the cDNA clones that we have sequenced.

    Other Cryptococcus neoformans Sites


    Acknowledgment

    Should you find this data useful and wish to reference it, please acknowledge the Cryptococcus neoformans cDNA Sequencing Project, NIH-NIAID grant number AI147079 and Bruce A. Roe, Doris Kupfer, Heather Bell, Sun So, Yuong Tang, Jennifer Lewis, Sola Yu, Kent Buchanan, Dave Dyer and Juneann Murphy; this project is supported by NIH-NIAID grant number AI147079.

    If possible, please send us both the literature citation once published, and a copy of the manuscript.

    Thanks, Bruce Roe, Doris Kupfer, Heather Bell, Sun So, Yuong Tang, Jennifer Lewis, Sola Yu, Kent Buchanan, Dave Dyer and Juneanne Murphy.
    The University of Oklahoma, Department of Chemistry and Biochemistry, Norman, Oklahoma 73019 and the University of Oklahoma Health Sciences Center, Department of Microbiology and Immunology, Oklahoma City, Oklahoma.

    Bruce Roe, broe@ou.edu

    Juneann Murphy, Juneann-Murphy@ouhsc.edu
    Kent Buchanan, kbuchan@mailhost.tcs.tulane.edu
    Dave Dyer, dyer@microbiology.bmsb.uokhsc.edu

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    Bruce Roe, broe@ou.edu