Fiddler Crab Celuca pugilator Transcriptome Analysis: Ecdysteroid Control of Limb Regeneration

Sanger Sequencing Data

Older cDNA clonal analyses

We have previously generated cDNA libraries for early blastemal stages of limb regeneration in Bluescript vectors. The early blastemal cDNA clone dataset consists of 2309 ESTs representing 980,511 high quality read nucleotides.

  • Search the early blastemal (4 days post autotomy) Celuca pugilator cDNA sequence data using Blastall to retrieve individual cDNA clones with their library identifier

  • Perform a keyword search of the results of blastn, blastx, tblastx search of the GenBank databases using the early blastemal Celuca pugilator cDNA unigene database.

    For Celuca pugilator enter your keyword here:

    Obtaining early blastemal (4 days post autotomy) Celuca pugilator data via ftp

  • Fiddler crab early blastemal cDNA Sequencing Data via ftp
  • Fiddler crab early blastemal unigen data via ftp
  • Fiddler crab early blastemal unigen BlastX data via ftp

    Late proecdysial limb bud Celuca pugilator cDNA

    We also have sequenced over 1,192 cDNA clones from a late proecdysial limb bud Celuca pugilator cDNA library. This dataset consists of 1337 ESTs representing 706,409 high quality read nucleotides.

  • Search the late proecdysial limb bud Celuca pugilator cDNA sequence data using Blastall to retrieve individual cDNA clones with their library identifier
  • Perform a keyword search of the results of blastn, blastx, tblastx search of the GenBank databases using the late pre-molt Celuca pugilator cDNA unigene database.

    For Celuca pugilator enter your keyword here:

    Obtaining late proecdysial limb bud Celuca pugilator cDNA via ftp

  • Fiddler crab late proecdysial limb bud cDNA Sequencing Data via ftp
  • Fiddler crab late proecdysial limb bud unigen data via ftp
  • Fiddler crab late proecdysial limb bud unigen BlastX data via ftp

    COMBINED Celuca pugilator cDNA sequence data

  • Search the combined limb bud Celuca pugilator cDNA sequence data using Blastall to retrieve individual cDNA clones with their library identifier
  • Perform a keyword search of the results of BlastX search of the GenBank nr database using the COMBINED Celuca pugilator regenerating limb bud cDNA unigene database.

    For Celuca pugilator enter your keyword here:

    Obtaining COMBINED Celuca pugilator cDNA data via ftp

  • Fiddler crab COMBINED cDNA sequence Data via ftp
  • Fiddler crab COMBINED unigen sequence data via ftp
  • Fiddler crab COMBINED unigen BlastX data via ftp

    Acknowledgment

    Should you find this data useful and wish to reference it, please acknowledge Ruihua Shi, Liping Zhou, Fares Najar, Hongshing Lai, Bruce Roe in the Department of Chemistry and Biochemistry, and Sunetra Das and David Durica in the Department of Biology at the University of Oklahoma. This work was funded in part by by a grant from the Oklahoma Center for the Advancement of Science and Technology (HR08-023) to David Durica.

    If possible, please send us both the literature citation once published, and a copy of the manuscript.

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    Bruce Roe, broe@ou.edu