Fungal (Aspergillus nidulans, Aspergillus parasiticus and Neurospora crassa) Cosmid and cDNA Sequencing


Fungal Genomic (Cosmid Cloned) DNA Sequencing

Aspergillus nidulans Cosmids

  • Cosmid swe06e08 - AC000133
  • Cosmid 30b1 - AC004395
  • Cosmid 2h2 - AC005299

    Aspergillus parasiticus Cosmids

  • Cosmid ap0 - AC005991
  • Cosmid pyj7 - AC007872

    Neurospora crassaCosmids

  • Cosmid nc14 - AC007959
  • Cosmid nc17 - AC007960
  • Cosmid nc19 - AC025046

    Fungal (Aspergillus nidulans, Aspergillus flavus and Neurospora crassa) cDNA Sequencing


    To date, we have isolated over 14,900 cDNA clones from a mixed vegatative and 24 hour asexual development culture of Aspergillus nidulans Strain FGSC A26(biA1) constructed in lambda zap by Dr. Rodolfo Aramayo, at Texas A&M University, College Station, Texas raramayo@bio.tamu.edu and over 6000 cDNA clones from an evening Neurospora crassa cDNA library and over 7000 cDNA clones from a morning cDNA library, constructed by Jay Dunlap at Dartmouth College, New Hampshire Jay.C.Dunlap@Dartmouth.edu
    We recently also have sequenced ca. 1400 cDNA clones from a wild type, Aspergillus flavus vegetative mycelia cDNA library created by Nancy Keller at Department of Plant Pathology, Texas A&M University who recently moved to the Department of Plant Pathology, University of Wisconson-Madison npk@plantpath.wisc.edu
    We recently also have sequenced ca. 1500 cDNA clones from a wild type, Neurospora crassa perathecia (fruting bodies) from a cDNA library created by Mary Ann Nelson at Department of Biology, University of New Mexico, Albuquerque, New Mexico manelson@unm.edu from RNA isolated five days after fertilization.

    Aspergillus nidulans Eighth Data Release - March 11, 1999

    Aspergillus flavus First Data Release - February 22, 2001

    Neurospora crassa Fifth Data Release - December 16, 1999

    Neurospora crassa perathecia cDNAs - First Data Release Dec. 19, 2000


    Search the Aspergillus nidulans or Neurospora crassa cDNA sequence data



    Obtaining the sequence data via ftp

    All of the cDNA sequence data we have obtained to date is available from our ftp site directly, by anonymous ftp to ftp.genome.ou.edu (logon as anonymous with password your e-mail address), cd to /pub/asper or /pub/nc_evening or /pub/nc_morning or /pub/nc_combined and get either the ascii data file called asper_est_with_anno.fa or asper_est_wo_anno.fa, or nc_evening_est_with_anno.fa or nc_evening_est_wo_anno.fa or nc_morning_est_with_anno.fa or nc_morning_est_wo_anno.fa or nc_all_est_wo_anno.fa files contains all the EST's sequenced to date concatenated into a flat file either with or without annotation, respectively.

  • Aspergillus nidulans Sequencing Data via ftp
  • Aspergillus flavus Sequencing Data via ftp
  • Neurospora crassa Evening cDNA Library EST Sequencing Data via ftp
  • Neurospora crassaMorning cDNA Library EST Sequencing Data via ftp
  • Neurospora crassa combined morning and evening cDNA Library EST Sequencing Data via ftp
  • Neurospora crassa perathecia cDNA Library EST Sequencing Data via ftp

    Clarification of our naming convention

    For Aspergillus nidulans For Neurospora crassa For Neurospora crassa perathecia

    Clone Availability

    At the present time, many of our cDNA clones are available through the Fungal Genetics Stock Center. Since not all the clones have been deposited at the FGSC yet, if you cannot find the clone you are interested in there, please contact us directly and we will make it available to you.

    Other Sites of Related Interest

  • Rolf Prade's Web Page at Oklahoma State Univ.
  • Aspergillus nidulans Genomic Home Page at Aspergillus-Genomics.org
  • Mycology: The Study of Fungi
  • The Neurospora Genome Project at Univ. New Mexico
  • Fungal Genome Resource at the University of Georgia
  • Candida albicans information

    Acknowledgment

    Should you find this data useful and wish to reference it, please acknowledge the Aspergillus nidulans and the Neurospora crassa cDNA Sequencing Project, and B. A. Roe, D. Kupfer, H. Zhu, J. Gray, S. Clifton, R. Prade, J. Loros J. Dunlap, M. Nelson; this project is supported by funds from the NSF-EPSCoR program.

    If possible, please send us both the literature citation once published, and a copy of the manuscript.

    Thanks, Bruce Roe, Doris Kupfer, Hua Zhu, Jennifer Gray, Sandy Clifton, Rolf Prade, Jennifer Loros, Jay Dunlap and Mary Anne Nelson.
    The University of Oklahoma, Department of Chemistry and Biochemistry, Norman, Oklahoma 73019, Oklahoma State University, Department of Microbiology and Molecular Genetics, Stillwater, Oklahoma, Dartmouth College, Hanover, New Hampshire, and Department of Biology, University of New Mexico, Albuquerque, New Mexico.


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    Bruce Roe, broe@ou.edu