Check below that the parameters are correct. If you have opened the gel for the first time, these numbers are taken from the the configuration file. If all gels for the current project use the same parameters, and so does this incoming gel, these parameters should be correct first time.
If you still need to change them, click on the text entry field of the parameter you want to change and correct the content using normal editing keystrokes. When you hit RETURN, while editing the parameters, the next text entry field will become active, so the cursor loops through these fields.
The standard file and the vector file can be set by clicking on the [file] button, which open a file chooser as a convenient tool to find and select files
If this gel hasn't been lane tracked before, it'll run the lane tracker before entering Step 2. When you hit [NEXT >>], the button will go black and the program goes away thinking. Step 2 will come up as soon as the lane tracking has finished successfully. While the lane tracker runs the main window freezes and waits for it to finish.
However you can run this and all other processing functions as a background job. Just switch on the batch mode by highlighting the [batch] button at the top.
When you click on [NEXT >>] now, the display won't freeze - it'll submit the lane tracking as a background job and return to the first chooser screen, where the gel will come up locked, with a little circle in the lane tracking column.
You can change any of the parameters. A description of all of the parameters is given in the help for the project defaults screen.