Mouse Genomic Sequencing
at the
University of Oklahoma

Sequencing Regions of the Mouse Genome Syntenic to Human Chromosome 22

NCBI Whole Genome Shotgun Database presently available from Mouse and Rat

Mouse trace database Mega BLAST search at the NCBI

EMBL SyntenyView of the mouse genomic regions syntenic to human chromosome 22

Mouse Genome Resources at the NCBI

  • NHGRI Press Release and list of Grant Awardees - MOUSE: NEXT IN LINE FOR DNA SEQUENCING

  • Our initial sequencing strategy is to sequence genomic BACs from the RPCI-23 Black 6 mouse library generated by Pieter deJong and collegues at the Department of Cancer Genetics at Roswell Park Cancer Institute in Buffalo, New York. The BACPAC Resource Center has relocated to the Children's Hospital Oakland Research Institute in Oakland, Ca., USA. Their web site is http://bacpac.chori.org/

  • We also are sequencing other regions of the mouse genome of high biological interest.

  • All of the RPCI-23 Female Mouse BACs that we are sequencing are from the NHGRI Mouse Genome Project approved C57BL/6J strain, aka Black 6 and requests for them should be directed to Dr. de Jong's BACPAC Resource Center at http://bacpac.chori.org/order.php

  • The BAC end sequences of 170,634 clones from the entire library RPCI-23 from C57BL/6J are available at the TIGR Mouse BAC end web site:
    http://www.TIGR.org/tdb/bac_ends/mouse/bac_end_intro.html

  • A table viewing the colones being sequenced of High Biomedical Interest can be viewed at URL: http://www.genome.ou.edu/MouseClones96a.html
    and the progress on sequencing these clones can be viewed at URL: http://www.genome.ou.edu/mus_totals.html

  • The Sanger Center has additional information and analysis of the regions of the mouse genome that are orthologous (aka syntenic) to human chromosome 22 on their Human Chromosome 22 Mouse Synteny web site.

  • Summary of the mouse libraries available from the BACPAC Resource Center.

    • RPCI - 21 Female (129S6/SvEvTac) Mouse PAC Library Female 129S6/SvEvTacÊ mouse spleen genomic DNA (partially MboI digested) was cloned into the Bam HI sites of the pPAC4 vector. The ligation products were transformed into DH10B electrocompetent cells (BRL Life Technologies).
    • RPCI - 22 (129S6/SvEvTac) Mouse BAC Library Female 129S6/SvEvTac (Taconic) mouse spleen genomic DNA was isolated and partially digested with a combination of EcoRI and EcoRI Methylase.Ê Size selected DNA was cloned into the pBACe3.6 vector at the EcoRI sites.Ê The ligation products were transformed into DH10B electrocompetent cells (BRL Life Technologies).
    • RPCI - 23 Female (C57BL/6J) Mouse BAC Library Female C57BL/6J mouse kidney and/or brain genomic DNA was isolated and partially digested with a combination of EcoRI and EcoRI Methylase.Ê Size selected DNA was cloned into the pBACe3.6 vector at the EcoRI sites.Ê The ligation products were transformed into DH10B electrocompetent cells (BRL Life Technologies).

  • Mouse libraries available from Invitrogen
    • Mouse clones from the Cal. Tech. library (given the prefix 'CT') are available from Invitrogen

  • Recently we have begun investigating a PCR-based approach using human chromosome 22 exon-specific primers for both PCR and custom synthetic primer-based sequencing. Details

  • We also are sequencing regions of other human and syntenic mouse chromosomes of high biological interest mapped by other collaborators. View a picture of the Human-Mouse Chromosome Homologies

  • In accord with the Bermuda Agreement and NHGRI policy, we are depositing all our human genomic sequence data into the High-Throughput Genomic Sequences (HTGS) division of GenBank as soon as a target large insert clone sequencing project can be assembled into contigs greater than 2 kb.

  • View a computer generated listing of all our mouse genomic DNA sequencing projects.

Search the Mouse Genome sequence data

Other Mouse Genome Related Links:

Home Page

Bruce Roe, broe@ou.edu