Staphylococcus aureus NCTC 8325 Genome Sequencing

The Sanger Centre is sequencing the epidemic MRSA strain EMRSA-16, and a hyper-virulent community acquired MSSA strain and is available at the Sanger Centre Staphylococcus aureus web site, the Institute for Genome Research (TIGR) is sequencing Staphylococcus aureus strain COL and their data is available from the TIGR Microbial Database, and the genomes of Staphylococcus aureus strains N315 and Mu50 have been sequenced at Kyushu Univ. in a joint project with Juntendo Univ. and recently published by M. Kuroda et al. in Lancet (M. Kuroda et al. (2001) The Lancet, 357, 1225-1240.).


We also have obtained several genomic BAC clones from Dr. Iandolo's group, which were generated by Dr. Veronica Worrell, and have begun sequencing them to aid in the final genomic sequence validation. These BACs are being sequenced by Linda Ray, Phoebe, Sulan, and Bart. This data will be available directly from the links below once they have been assigned GenBank Accession Numbers.

  • View the Latest Phrap High Stringency Staphylococcus aureus Statistics

    • Search the Staphylococcus aureus Genome sequence data

    Obtaining the sequence data via ftp

    All of our shotgun sequence data is available via ftp (see below) but at the present time, the various contigs are in "random" order and do not correspond to any specific relationship one to another. Please be aware that the order and contig number will change with each new release of data.

    Caution: the data has been neither proof read nor edited and thus should be considered preliminary raw sequence data. Also, because the sequence assembly program, Phrap, has the tendency to include inaccurate sequence reads on the ends of contigs, be cautious when using sequences within 1-200 bases of the ends of individual contigs. Please take this into account when using this data

    If you have determined which contig is of interest to you using the above Blast search capability on the OU Server, this contig(s) are in the 'staph-1k.fa' file or the 'contigs-1k' directory on our ftp site.

    If however, you determined which contig was of interest using the 'KeyWord Search' option, then the contig(s) of interest will be found in the 'staph_weekly-1k.fa' file or the 'contigs_weekly-1k' directory

  • Obtaining the Staphylococcus aureus Sequencing Data via ftp

    • If instead, you would rather ftp this file directly, you can ftp to ftp.genom.ou.edu (logon as anonymous with password your e-mail address). Read the 00readme file in the ftp directory to determine which files you are interested in.

    Clone Availability

    The Staphylococcus aureus strain NCTC 8325 is available from Dr. John Iandolo who maintains a large collection of marked S. aureus strains (more that 4000) and enquiries about obtaining this and other Staphylococcus aureus strains should be directed to him. At the present time, none of the shotgun clones are available for distribution. We anticipate at some point to generate a set of ordered sub-clones that will be made available throught the ATCC.

    Acknowledgment

    Should you find this data useful and wish to reference it, please acknowledge the Staphylococcus aureus Genome Sequencing Project, and B.A. Roe, Y. R. Tian, H. Jia, S. Li, S. Lin, S. Kenton, H. Lai, JD White, A. Dorman, F. Z. Najar, S. Clifton, V. Worrell and J. Iandolo with funding from the NIH and the Merck Genome Research Institute.

    If possible, please send us both the literature citation once published, and a copy of the manuscript.

    Thanks, Bruce Roe, Runying Tian, HongGui Jia, YuDong Qian, Shaoping Lin, Suling Li, A. Dorman, Steve Kenton, HongShing Lai, Jim D. White, F. Z. Najar, Sandy Clifton, Veronica Worrell and John Iandolo.
    The University of Oklahoma, Department of Chemistry and Biochemistry, Norman, Oklahoma 73019 and The University of Oklahoma Health Sciences Center, Department of Microbiology and Immunology, Oklahoma City, Oklahoma 73190

    John Iandolo, John-Iandolo@ouhsc.edu

    Bruce Roe, broe@ou.edu
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    Bruce Roe, broe@ou.edu