Streptococcus pyogenes Genome Sequencing
Strain M1 GAS

The sequence of the Streptococcus pyogenes Strain M1 GAS genome has been published. J.J. Ferretti, W.M. McShan, D. Ajdic, D. J. Savic, G. Savic, K. Lyon, S. Sezate, A. N. Suvorov, C. Primeaux, S. Kenton, H. Lai, S. Lin, Y. Qian, H. Jia, H. Zhu, Q. Ren, F.Z, Najar, L. Song, J. White, X. Yuan, S. W. Clifton, B. A. Roe, R. McLaughlin. Complete Genome Sequence of an M1 Strain of Streptococcus pyogenes Proc. Natl. Acad. Sci. USA. 98, 4658-4663 (2001). Obtain a PDF version of this publication

The Streptococcus pyogenes sequence with annotation has GenBank accession number AE004092.

This joint project being completed as a collaboration between our laboratory at the University of Oklahoma, Norman Campus's Advanced Center for Genome Technology, in the Department of Chemistry and Biochemistry, which is funded in part by an NSF EPSCoR grant, and Dr. Joseph Ferretti's laboratory in the Department of Microbiology and Immunology at the University of Oklahoma Health Sciences Center. This project is funded by USPHS NIH grant #AI38406.


Streptococcus pyogenes Genome Sequencing Progress

  • To date, we have sequenced over 16,330 individual, shotgun-based, double stranded templates generated from a randomly sheared library of the Streptococcus pyogenes genome, with both the universal forward and reverse pUC sequencing primers.
  • Over 14 million bases of sequence have been collected and aligned into a single large contiguous sequence of 1,852,442 bases for the entire Streptococcus pyogenes genome.
  • Final sequence proofreading was accomplished by direct sequencing off large insert lambda clones, PCR products from genomic templates, and primer walking-based sequencing off large (4-8Kb) plasmid sub-clones.

    Sequence Annotation

    In collaboration with Evengi Selkov and Ross Overbeek at Argonne National Labs, we now are providing a WIT2 analysis of the potential reading frames and an overview of the Streptococcus pyogenes metabolic pathways.


    Search the Streptococcus pyogenes Genome sequence data


    Obtaining the sequence data via ftp

    All of our shotgun sequence data is available via ftp (see below).

    The latest sequence data is available in the 'strep-2k.fa' file on our ftp site.

  • Obtaining the Streptococcus pyogenes Sequencing Data via ftp
  • The Streptococcus pyogenes sequence with annotation has been submitted to GenBank and given accession number AE004092.


    If instead, you would rather ftp this file directly, you can ftp to ftp.strep.ou.edu (logon as anonymous with password your e-mail address). Read the 00readme file in the ftp directory to determine which files you are interested in.

    Clone Availability

  • The Streptococcus pyogenes - Strain M1 GAS being sequenced, has been submitted to the ATCC and given accession number ATCC 700294.
  • At the present time, none of the shotgun clones are available for distribution. We anticipate at some point to generate a set of ordered sub-clones that will be made available throught the ATCC.

    Literature Reference Describing Strain being sequenced

  • Suvrov, A.N. and Ferretti, J.J. Physical and Genetic Map of an M type 1 strain of Streptococcus pyogenes. J. Bacteriology 178, 5546-5549 (1996).

    Acknowledgment

    Should you find this data useful and wish to reference it, please acknowledge the Streptococcal Genome Sequencing Project funded by USPHS/NIH grant #AI38406 and B. A. Roe, S. P. Linn, L. Song, X. Yuan, S. Clifton, R. E. McLaughlin, M. McShan and J. Ferretti.

    If possible, please send us both the literature citation once published, and a copy of the manuscript.

    Thanks, Bruce Roe, ShaoPing Linn, Lin Song, XiLing Yuan, Sandy Clifton, Bob McLaughlin, Mike McShan and Joseph Ferretti.
    The University of Oklahoma, Department of Chemistry and Biochemistry, Norman, Oklahoma 73019 and The University of Oklahoma Health Sciences Center, Department of Microbiology and Immunology, Oklahoma City, Oklahoma 73190

    Joe Ferretti, Joseph-Ferretti@uokhsc.edu

    Bob McLaughlin, bob-mclaughlin@ouhsc.edu
    Mike McShan, wmcshan@REX.RE.uokhsc.edu
    Bruce Roe, broe@ou.edu

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    Bruce Roe, broe@ou.edu