Amblyomma americanum (lone star tick) cDNA Sequencing
Doris Kupfer, Sara Downard, and Laura Hern in our Genome Center
at the University of Oklahoma, have sequenced the 3' and/or 5' ends
of double stranded cDNAs constructed from the salivary glands of
the lone star tick, Amblyomma americanum constructed by
Richard Essenberg and Majd Aljamali, Department of Biochemistry
and Molecular Biology at Oklahoma State University, Stillwater OK.
Four libraries are included in this August 27, 2003 release.
- Lib1:TSG Lib1, female, salivary gland, 50-200 mg feeding adults
- Lib2:TSG Lib2, female, salivary gland, unfed adults, feeding adults, replete adults
- Lib3:TSG Lib3, female, salivary gland, 3 day feeding adults
- Lib4:TSG Lib4, female, salivary gland, 50-200 mg feeding adults, was
constructed from TSG Lib1 by normalization following procedure of, Bonaldo et al
(Genome Res. 1996 Sep;6(9):791-806).
Amblyomma americanum lone star tick
Latest Data Release - August 27, 2003 1757 total ESTs that were
assembled into 810 entries and 608,477 nucleotides
in the four separately libraries and combined into one searchable database.
- The Amblyomma americanum
lone star tick salivary gland cDNA libraries Lib1, Lib2 and Lib4 were constructed
from multiple feeding stages in Clonetech pTriplEx2 vector by directionally cloning into
the 5' EcoRI and 3' SfiIb sites. Lib3 was constructed from 3 day feeding adults by
Stratagene, Inc. into pBlueScript SK- .
- Ribosomal RNA, mitochondrial RNA, vector and small
(less than 100bp) inserts, will be removed prior to assembly of a UniGene database using Phred (Phil Green, University of Washington) .
- All of our data is available from our ftp site, and we now have added the ability
to perform blast searches on this data. A keyword search of a blastx search of GenBank with this data also is available.
Search the Amblyomma americanum cDNA sequence data
Obtaining the sequence data via ftp
All of the cDNA sequence data we have obtained to date is available from our
ftp site directly, by anonymous ftp to ftp.genome.ou.edu (logon as anonymous with
password your e-mail address), cd to /pub/tick_est and get the ascii data file
called Liball.fa or lt_est_wo_anno.fa. These files contains all
the EST's sequenced to date concatenated into a flat file.
Amblyomma americanum Sequencing Data via ftp
Clarification of our naming convention
For Amblyomma americanum
- Since at present, we only are determining 5' end sequences,
to be consistent with our other EST projects, all these clones
have the notation .r1 at the end of the clone name.
At the present time, none of the cDNA clones are available for distribution.
We anticipate at some point to generate a set of arrayed cDNA clones that will be made available through the ATCC.
Should you find this data useful and wish to reference it, please acknowledge
the Amblyomma americanum cDNA Sequencing Project,
and Bruce A. Roe, Doris Kupfer, Sara Downard, Laura Hern, Majed Aljamali, and Richard Essenberg; this
project is supported by funds from the University of Oklahoma, Oklahoma State University and the NSF-EPSCoR program.
If possible, please send us both the literature citation once published,
and a copy of the manuscript.
Thanks, Bruce Roe, firstname.lastname@example.org
Doris Kupfer, email@example.com
Richard Essenberg, firstname.lastname@example.org
Bruce Roe, Doris Kupfer, Sara Downard, Laura Hern, Majd Aljamali and Richard Essenberg.
The University of Oklahoma, Department of Chemistry and Biochemistry, Norman,
Oklahoma, 73019 and the Department of Biochemistry and Molecular Biology,
Oklahoma State University, Stillwater, Oklahoma, 74078.
Bruce Roe, email@example.com