Amblyomma americanum (lone star tick) cDNA Sequencing


Doris Kupfer, Sara Downard, and Laura Hern in our Genome Center at the University of Oklahoma, have sequenced the 3' and/or 5' ends of double stranded cDNAs constructed from the salivary glands of the lone star tick, Amblyomma americanum constructed by Richard Essenberg and Majd Aljamali, Department of Biochemistry and Molecular Biology at Oklahoma State University, Stillwater OK.

  • Four libraries are included in this August 27, 2003 release.

    Amblyomma americanum lone star tick Latest Data Release - August 27, 2003 1757 total ESTs that were assembled into 810 entries and 608,477 nucleotides in the four separately libraries and combined into one searchable database.


    Search the Amblyomma americanum cDNA sequence data

    Obtaining the sequence data via ftp

    All of the cDNA sequence data we have obtained to date is available from our ftp site directly, by anonymous ftp to ftp.genome.ou.edu (logon as anonymous with password your e-mail address), cd to /pub/tick_est and get the ascii data file called Liball.fa or lt_est_wo_anno.fa. These files contains all the EST's sequenced to date concatenated into a flat file.

  • Amblyomma americanum Sequencing Data via ftp

    Clarification of our naming convention

    For Amblyomma americanum
    • Since at present, we only are determining 5' end sequences, to be consistent with our other EST projects, all these clones have the notation .r1 at the end of the clone name.

    Clone Availability

    At the present time, none of the cDNA clones are available for distribution. We anticipate at some point to generate a set of arrayed cDNA clones that will be made available through the ATCC.

    Acknowledgment

    Should you find this data useful and wish to reference it, please acknowledge the Amblyomma americanum cDNA Sequencing Project, and Bruce A. Roe, Doris Kupfer, Sara Downard, Laura Hern, Majed Aljamali, and Richard Essenberg; this project is supported by funds from the University of Oklahoma, Oklahoma State University and the NSF-EPSCoR program.

    If possible, please send us both the literature citation once published, and a copy of the manuscript.

    Thanks,
    Bruce Roe, Doris Kupfer, Sara Downard, Laura Hern, Majd Aljamali and Richard Essenberg. The University of Oklahoma, Department of Chemistry and Biochemistry, Norman, Oklahoma, 73019 and the Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma, 74078.

    Bruce Roe, broe@ou.edu
    Doris Kupfer, dkupfer@ou.edu
    Richard Essenberg, ressenberg@biochem.okstate.edu
    Home Page

    Bruce Roe, broe@ou.edu